This study thatĀ evaluated the potential use of mosquitoes for surveillance, included feeding of MERS virus to Anopheles gambiae mosquitoes. To date, only one report related to epidemic coronaviruses and mosquitoes has been published 16. In spite of the recovery of coronavirus or coronavirus-like agents from various arthropods 14, 15, no virus in the family has been isolated from mosquitoes. Similar to over 500 viruses that are transmitted by arthropods 13, with the exception of African swine fever virus, coronaviruses have an RNA genome. The use of intrathoracic inoculation, also addressed published reports that the natural physical breaching of the midgut wall by filarial, may enable a disseminated coinfection of viruses in resistant mosquitoes 12. These include not only non-hematophagous mosquitoes such as Toxorhynchites spp, but also male mosquitoes and even beetles and butterflies 10, 11. Intrathoracic inoculation 8, 9 of virus directly into the hemocoel can accomplish short-term infection of insects that could never be naturally infected because they do not feed on blood. If these barriers are bypassed by direct inoculation of virus into the hemocoel, then even non-susceptible mosquitoes can be infected. Overcoming the midgut infection and escape barriers is essential for a virus to be transmissible by mosquitoes. To be a biological vector of viruses, mosquitoes must take up sufficient virus to infect midgut epithelial cells, and the virus must then disseminate to infect other organs in the hemocoel, notably the salivary glands. Despite the lack of detectable viremia, experiments to determine the potential role of mosquitoes in SARS-CoV-2 transmission, are necessary because previous experiments have demonstrated that mosquitoes may become infected with viruses even when exposed to levels of infectious virus that are below the level of detection 5, 6, 7. Although we do not know the duration of virus infectivity on contaminated surfaces, mechanical transmission by non-hematophagous arthropods seems highly unlikely, and even if not impossible, would result in very few, if any human infections, and not be epidemiologically relevant. Since mechanical transmission of viruses by arthropods requires a very high viremia 4, even if mosquitoes were interrupted when feeding on a SARS-CoV-2 infected person, the mouthparts would not be contaminated. Lack of viremia is also suggested by the fact that neither SARS-CoV nor MERS infections have resulted from blood transfusions or organ transplantations. Recent studies with infected humans and non-human primates infected with SARS-CoV-2, found no detectable virus in peripheral blood 2, 3. For example, neither the closely related SARS-CoV nor MERS produce the level of virus in the blood that for typical arthropod-borne viruses such as dengue and yellow fever, would be regarded as high enough to infect mosquitoes. The presumption may be based on various observations and facts extrapolated from other coronaviruses. The WHO has definitively stated that mosquitoes cannot transmit the virus 1, and in interviews, various experts have unanimously and definitively also dispelled the suggestion that SARS-CoV-2 could be transmitted by mosquitoes. The question has been asked as to whether or not SARS-CoV-2, the causative agent of COVID-19, can infect and be transmitted by mosquitoes.